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Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi

Received: 8 October 2015     Accepted: 19 October 2015     Published: 13 November 2015
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Abstract

Antioxidant activity test on protein extract of Kerang Kepah (Atactodea striata) by scavenging method of free radical of DPPH (N, N-diphenyl-N-picrylhydrazyl) has been carried out. This study was conducted to determine the antioxidant activity and the amino acid composition of the protein extract of Atactodea striata. The analysis results showed that the protein extract of Atactodea striata has the most complete amino acid composition and the highest antioxidant activity of the protein fraction at a rate of 30-50% ammonium sulfate saturation with IC50 value of 183.75µg/mL. However, when it is compared to the antioxidant activity of ascorbic acid (IC50 values = 2.51µg/mL), the antioxidant activity of the protein extract fraction of Atactodea striata is still much lower.

Published in American Journal of Biomedical and Life Sciences (Volume 3, Issue 6)
DOI 10.11648/j.ajbls.20150306.12
Page(s) 111-114
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Atactodea striata, Antioxidant, DPPH, HPLC

References
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[4] R.D. Barnes, Invertebrate Zoology, Saunders College, Fourth Edition, 1982.
[5] L. Nurjanah, D. Hardjito, Monintya, M. Bintang, and D.R. Agungpriyono,“Aktivitasantioksidanlintahlaut (Discodorissp) dariperairan Pulaulintahlaut dariperairan Pulau Buton Sulawesi Tenggara,” Prosiding Seminar Nasional Pengolahan Produkdan Bioteknologi Kelautandan Perikanan. Jakarta, 2009.
[6] A. Nurjanah, Abdulla, and A. Apriand, “Aktivitas antioksidan dan komponen bioaktif pada keong ipong-ipong (Fasciolaria salmo),”J. Pengolahan Hasil Perikanan Indonesia, vol.14, pp 22-29, 2011.
[7] C. Jiang, M. Wang, J. Lin, D. Gan, X. Zeng, “Extraction, preliminary characterization, antioxidant and anticancer activities in vitro of polysaccharides from Cyclinosinensis,” Carbohydrate Polymers, vol 84, pp. 851-857, 2011.
[8] D.Y. Zhou, B.W. Zhu, L. Qiao, H.T. Wu, D.M. Li, J.F. Yang and Y. Murata, “In vitro antioxidant activity of enzymatic hydrolysates prepared from abalone (Haliotis discus hannaiIno) viscera,” Food and Bioproducts Processing, 2011.
[9] V.N. Madhu, K. Sivaperumal, K. Kamala and B.G. Kulkarni, “Antibacterial and Antioxidant Activities of The Tissue Extract of Verna viridis From Versova Coast,” International Journal of Pharmacy and Pharmaceutical Sciences, Mumbai, 2014.
[10] L. Huang, Protein dalam Air Mata Obat untuk AIDS, (On line), (http://www.rad .net.id/warta/wao4701.htm, (diakses 2 Januari 2013), 1999.
[11] Nollet, M. L. Leo, “Handbook of Food Analysis and Amino Acid,” 1996.
[12] D.T. Plummer, “An Introduction to Practical Biochemistry,” Second Edition. Tata Mc. Graw Hill Publishing Company Ltd. New Delhi, 1979.
[13] R.K. Scopes, “Protein Purification Principles and Ptactice,” Second edition, Springger-Verlag, New York, 1987.
[14] Liyana, F. Pathiranam, Shahidi,” Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric pH conditions,” J. Agric. Food Chem. Vol. 53, pp. 2430-2440. 2005.
[15] W. Moka,“Identifikasi dan Inventarisasi Jenis Kerang Laut yang Digunakan Sebagai Obat Tradisonal di Sulawesi Selatan”, UNHAS, Makassar, 1982.
Cite This Article
  • APA Style

    Tahirah Hasan, Abdul Wahid Wahab, Natsir Djide, Muhammad Zakir. (2015). Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi. American Journal of Biomedical and Life Sciences, 3(6), 111-114. https://doi.org/10.11648/j.ajbls.20150306.12

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    ACS Style

    Tahirah Hasan; Abdul Wahid Wahab; Natsir Djide; Muhammad Zakir. Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi. Am. J. Biomed. Life Sci. 2015, 3(6), 111-114. doi: 10.11648/j.ajbls.20150306.12

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    AMA Style

    Tahirah Hasan, Abdul Wahid Wahab, Natsir Djide, Muhammad Zakir. Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi. Am J Biomed Life Sci. 2015;3(6):111-114. doi: 10.11648/j.ajbls.20150306.12

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  • @article{10.11648/j.ajbls.20150306.12,
      author = {Tahirah Hasan and Abdul Wahid Wahab and Natsir Djide and Muhammad Zakir},
      title = {Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {3},
      number = {6},
      pages = {111-114},
      doi = {10.11648/j.ajbls.20150306.12},
      url = {https://doi.org/10.11648/j.ajbls.20150306.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20150306.12},
      abstract = {Antioxidant activity test on protein extract of Kerang Kepah (Atactodea striata) by scavenging method of free radical of DPPH (N, N-diphenyl-N-picrylhydrazyl) has been carried out. This study was conducted to determine the antioxidant activity and the amino acid composition of the protein extract of Atactodea striata. The analysis results showed that the protein extract of Atactodea striata has the most complete amino acid composition and the highest antioxidant activity of the protein fraction at a rate of 30-50% ammonium sulfate saturation with IC50 value of 183.75µg/mL. However, when it is compared to the antioxidant activity of ascorbic acid (IC50 values = 2.51µg/mL), the antioxidant activity of the protein extract fraction of Atactodea striata is still much lower.},
     year = {2015}
    }
    

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    T1  - Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi
    AU  - Tahirah Hasan
    AU  - Abdul Wahid Wahab
    AU  - Natsir Djide
    AU  - Muhammad Zakir
    Y1  - 2015/11/13
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajbls.20150306.12
    DO  - 10.11648/j.ajbls.20150306.12
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 111
    EP  - 114
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20150306.12
    AB  - Antioxidant activity test on protein extract of Kerang Kepah (Atactodea striata) by scavenging method of free radical of DPPH (N, N-diphenyl-N-picrylhydrazyl) has been carried out. This study was conducted to determine the antioxidant activity and the amino acid composition of the protein extract of Atactodea striata. The analysis results showed that the protein extract of Atactodea striata has the most complete amino acid composition and the highest antioxidant activity of the protein fraction at a rate of 30-50% ammonium sulfate saturation with IC50 value of 183.75µg/mL. However, when it is compared to the antioxidant activity of ascorbic acid (IC50 values = 2.51µg/mL), the antioxidant activity of the protein extract fraction of Atactodea striata is still much lower.
    VL  - 3
    IS  - 6
    ER  - 

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Author Information
  • Department of Chemistry, Graduate School of Science, Hasanuddin University, Makassar 90245, South Sulawesi, Indonesia

  • Department of Chemistry, Graduate School of Science, Hasanuddin University, Makassar 90245, South Sulawesi, Indonesia

  • Faculty of Pharmacy, Hasanuddin University, Makassar 90245, South Sulawesi, Indonesia

  • Department of Chemistry, Graduate School of Science, Hasanuddin University, Makassar 90245, South Sulawesi, Indonesia

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