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Laboratory Diagnostic Tools for Checking Ebola Viral Infections in Africa

Received: 24 December 2014     Accepted: 28 December 2014     Published: 15 February 2015
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Abstract

EBOV share many symptoms with a lot of common diseases, so only a well trained Laboratorian can properly collect samples, handle, do differential diagnosis and contain the disease. Quality assurance and control should be on-going in medical Laboratyories. BSL-4 laboratories should be built in African countries where this disease is prevalent. The government should develop a maintenance culture for the infrastructure. Maximum containment is needed when working with this virus as 90% of infected people die.

Published in American Journal of Clinical and Experimental Medicine (Volume 3, Issue 1-1)

This article belongs to the Special Issue Clinical Innovations, Developments in the Diagnosis, Management and Prevention of Ebola Disease (Marburg fever) and Hemorrhagic Fevers

DOI 10.11648/j.ajcem.s.2015030101.16
Page(s) 29-32
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Symptoms, Laboratories, Differential Diagnosis, Quality Assurance, BSL-4 Laboratory, Maintenance Culture, Maximum Containment

References
[1] Australian Public Health Laboratory Network: Laboratory Precautions for Samples Collected from Patients with Suspected Viral Haemorrhagic Fevers
[2] CDC: http://www.cdc.gov/vhf/ebola/hcp/interim-guidance-specimen-collection-submission-patients-suspected-infection-ebola.html
[3] CDC: Interim Guidance for Managing Patients with Suspected Viral Haemorrhagic Fever in U.S. Hospitals
[4] Ebola Virus 2014 West Africa Outbreak – CDC website link
[5] Borio, L., T. Inglesby, C. J. Peters, A. L. Schmaljohn, J. M. Hughes, P. B. Jahrling, T. Ksiazek, K. M. Johnson, A. Meyerhoff, T. O'Toole, M. S. Ascher, J. Bartlett, J. G. Breman, E. M. Eitzen, Jr., M. Hamburg, J. Hauer, D. A. Henderson, R. T. Johnson, G. Kwik, M. Layton, S. Lillibridge, G. J. Nabel, M. T. Osterholm, T. M. Perl, P. Russell, and K. Tonat. (2002). Hemorrhagic fever viruses as biological weapons: medical and public health management. JAMA 287:2391-2405
[6] Drosten, C., S. Gottig, S. Schilling, M. Asper, M. Panning, H. Schmitz, and S. Gunther. (2002). Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. J. Clin. Microbiol. 40:2323-2330
[7] Feldmann, H., S. Jones, H. D. Klenk, and H. J. Schnittler. (2003). Ebola virus: from discovery to vaccine. Nat. Rev. Immunol. 3:677-685
[8] Ikegami, T., M. Niikura, M. Saijo, M. E. Miranda, A. B. Calaor, M. Hernandez, L. P. Acosta, D. L. Manalo, I. Kurane, Y. Yoshikawa, and S. Morikawa. (2003). Antigen capture enzyme-linked immunosorbent assay for specific detection of Reston Ebola virus nucleoprotein. Clin. Diagn. Lab. Immunol. 10:552-557
[9] Leroy, E. M., S. Baize, C. Y. Lu, J. B. McCormick, A. J. Georges, M. C. Georges-Courbot, J. Lansoud-Soukate, and S. P. Fisher-Hoch. (2000). Diagnosis of Ebola haemorrhagic fever by RT-PCR in an epidemic setting. J. Med. Virol. 60:463-467.
[10] Lucht, A., R. Grunow, P. Moller, H. Feldmann, and S. Becker. (2003). Development, characterization and use of monoclonal VP40-antibodies for the detection of Ebola virus. J. Virol. Methods 111:21-28.
[11] Mitchell SW and McCormick JB, .J Clin. Microbiol. 1984, 20(3):486.)
[12] Niedrig, M., H. Schmitz, S. Becker, S. Gunther, J. ter Meulen, H. Meyer, H. Ellerbrok, A. Nitsche, H. R. Gelderblom, and C. Drosten. (2004). First international quality assurance study on the rapid detection of viral agents of bioterrorism. J. Clin. Microbiol. 42:1753-1755.
[13] Niikura, M., T. Ikegami, M. Saijo, I. Kurane, M. E. Miranda, and S. Morikawa. (2001). Detection of Ebola viral antigen by enzyme-linked immunosorbent assay using a novel monoclonal antibody to nucleoprotein. J. Clin. Microbiol. 39:3267-3271.
[14] Mitchell S.W and McCormick J.B (1984), .J Clin. Microbiol. 1984, 20(3):486.)
[15] Towner, J. S., P. E. Rollin, D. G. Bausch, A. Sanchez, S. M. Crary, M. Vincent, W. F. Lee, C. F. Spiropoulou, T. G. Ksiazek, M. Lukwiya, F. Kaducu, R. Downing, and S. T. Nichol. (2004). Rapid diagnosis of Ebola hemorrhagic fever by reverse transcription-PCR in an outbreak setting and assessment of patient viral load as a predictor of outcome. J. Virol. 78:4330-4341.
[16] www.cdc.gov/HAI/pdfs/bbp/VHFinterimGuidance05_19_05.pdf, accessed August 11, 2014)
Cite This Article
  • APA Style

    Polycarp Chia, Elvis Fon Tatah, Kenneth Yongabi. (2015). Laboratory Diagnostic Tools for Checking Ebola Viral Infections in Africa. American Journal of Clinical and Experimental Medicine, 3(1-1), 29-32. https://doi.org/10.11648/j.ajcem.s.2015030101.16

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    ACS Style

    Polycarp Chia; Elvis Fon Tatah; Kenneth Yongabi. Laboratory Diagnostic Tools for Checking Ebola Viral Infections in Africa. Am. J. Clin. Exp. Med. 2015, 3(1-1), 29-32. doi: 10.11648/j.ajcem.s.2015030101.16

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    AMA Style

    Polycarp Chia, Elvis Fon Tatah, Kenneth Yongabi. Laboratory Diagnostic Tools for Checking Ebola Viral Infections in Africa. Am J Clin Exp Med. 2015;3(1-1):29-32. doi: 10.11648/j.ajcem.s.2015030101.16

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  • @article{10.11648/j.ajcem.s.2015030101.16,
      author = {Polycarp Chia and Elvis Fon Tatah and Kenneth Yongabi},
      title = {Laboratory Diagnostic Tools for Checking Ebola Viral Infections in Africa},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {3},
      number = {1-1},
      pages = {29-32},
      doi = {10.11648/j.ajcem.s.2015030101.16},
      url = {https://doi.org/10.11648/j.ajcem.s.2015030101.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.s.2015030101.16},
      abstract = {EBOV share many symptoms with a lot of common diseases, so only a well trained Laboratorian can properly collect samples, handle, do differential diagnosis and contain the disease. Quality assurance and control should be on-going in medical Laboratyories. BSL-4 laboratories should be built in African countries where this disease is prevalent. The government should develop a maintenance culture for the infrastructure. Maximum containment is needed when working with this virus as 90% of infected people die.},
     year = {2015}
    }
    

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    AB  - EBOV share many symptoms with a lot of common diseases, so only a well trained Laboratorian can properly collect samples, handle, do differential diagnosis and contain the disease. Quality assurance and control should be on-going in medical Laboratyories. BSL-4 laboratories should be built in African countries where this disease is prevalent. The government should develop a maintenance culture for the infrastructure. Maximum containment is needed when working with this virus as 90% of infected people die.
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Author Information
  • Department of Biochemistry, Catholic University of Cameroon, Bamenda, Cameroon

  • Science for Life Foundation, Bamenda, Cameroon

  • Phyto-Biotechnology Research Foundation Institute, Catholic University of Cameroon, Bamenda, Cameroon

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